[[http://www.zeiss.com/4125681F004CA025/?Open|{{facilities:zeiss.jpg?80| }}]] ====== Zeiss TIRF System ====== {{facilities:tirf1aa.jpg?nolink&325|Zeiss TIRF System}} {{facilities:tirf4.jpg?nolink&183|Detail, laser safety cover}} (space) The **Zeiss Axiovert 200M** is a modern, motorized, general-purpose inverted microscope suitable for most non-confocal imaging applications. The system includes a digital camera and Axiovision software. It also includes the Total Internal Reflection Fluorescence **(TIRF)** hardware package from Zeiss, including a multi-line 100mW Lasos 77 Series laser that enables very high-resolution imaging of signals near biological membranes. This is a relatively new imaging modality that is likely to become widely used in both cell biology and neuroscience research applications. The TIRF system is located in the **Neuroscience Imaging Center**, room **E632c**. For additional information and training, contact Maryanne Pendergast at 368-2575. ===== ====== Personnel ====== ===== * **Maryanne Pendergast** \\ Training and Facility Manager \\ 368-2575 (office) \\ 216-224-8649 (cell)\\ [[mxp19@case.edu]] ===== Equipment ===== * Axiovert 200M inverted microscope \\ * 100mW multi-line (**458**nm, **488**nm, **514**nm) Lasos 77 Series laser\\ * Axiovision software ===== Fees ===== ^Widefield^TIRF^ | $15/ hr | $25/hr |