[[http://www.moleculardevices.com/pages/software/metamorph.html |{{facilities:metamorph.jpg?75| }}]] ====== Inverted Wide-field Microscope ====== {{facilities:meta_for_web.jpg?nolink&500|Widefield Metamorph system}} (space) The **Inverted Wide-Field Microscope** is located on the sixth floor of the Medical School in room **E630D**. The system integrates a Zeiss Axiovert 405 microscope with software, computers and cameras. The software, **Metamorph** and **Metafluor**, is designed to support image acquisition, morphometric analysis, calcium ratio imaging, 3D reconstruction, and time lapse experiments. A recent upgrade including a **Bioptechs Delta T4** stage and objective heater has greatly enhanced the live cell imaging and movie-making capabilities of the system. QuickTime and AVI movies created in Metamorph can now be exported into PowerPoint presentations. For additional information and training, please contact Maryanne Pendergast at X2575. ===== ====== Personnel ====== ===== * **Maryanne Pendergast** \\ Training and Facility Manager \\ 368-2575 (office) \\ 216-224-8649 (cell)\\ [[mxp19@case.edu]] ===== Equipment ===== * Zeiss Axiovert 405M inverted microscope. * Mercury and Xenon arc light sources. * Princeton Instruments Micromax cooled ccd (digital) camera. * 35mm and 4x5 format cameras. * Numerous objectives with long and short working distances. * Metamorph version 4.6.6. * Metafluor version 3.0. ===== Policies ===== * All users must be trained and approved for independent operation of the system by Maryanne. * Everyone is expected to use and care for the equipment as demonstrated during the initial training session. Abuse of the system may result in the cancellation of user privileges; repair costs will be charged to the lab responsible for damages. * Data can be stored short-term on the NIC server which is accessible to all computers within the facility as a mapped drive (Z:\). The server is available to other computers through a web interface. Data can also be saved to CDs and 100MB ZIP drives. * Sign up during the day is first come/ first serve and may be restricted to two half days per week during exceptionally busy times. * Sign-up should include user and PI names, time, and phone number. ===== Fees ===== ^Widefield^ | $15/ hr | ===== Useful Links ===== Web site for Molecular Devices, Inc. \\ http://www.moleculardevices.com/pages/software/metamorph.html Web site for Carl Zeiss, North America \\ http://www.zeiss.com/us/