{{facilities:biorad.jpg}} ====== iCycler Real-Time PCR System ====== {{facilities:icycler.jpg?375&nolink|iCycler real-time PCR system}} (space) The **Bio-Rad iCycler iQ** PCR detection system allows the user to both monitor and quantify a Polymerase Chain Reaction (PCR) in real time. A broad-spectrum tungsten light source and multiple filters permit a wide range of excitation and emission between 400-700nm, and also the multiplexing of up to four (4) different reporter fluorophors. On-line display allows visual confirmation of the PCR amplification in progress, and a 350,000 pixel CCD detector makes possible the simultaneous detection of up to 96 samples. The iCycler iQ is located in the Zigmond lab, room **E734A**. Please contact Rebecca Schreiber at x4615 for additional information. ===== ====== Personnel ====== ===== * **Dr. Richard Zigmond** \\ Professor \\ 368-4614 \\ [[rez@po.case.edu]] * **Rebecca C. Schreiber** \\ Training \\ 368-4615 \\ [[rcs519@case.edu]] ===== Equipment ===== * Bio-Rad iCycler iQ real time PCR system. * Dell 1.7 GHz Pentium PC with 512 MB RAM. * Printer. ===== Policies ===== * All prospective users must attend a Bio-Rad training workshop prior to using the system. * A copy of the user manual and software for data analysis is available through Rebecca Schreiber. * Signup for the equipment is first come/first serve. The sign-up calendar is located on the door of room E734A. * Data is stored on zip disks. * **The facility is not responsible for data storage and all data remaining on the hard drive is subject to immediate removal**. * **The facility is not responsible for providing chemical reagents and consumable supplies**. A list of the necessary reagents and supplies, however, may be obtained from Rebecca Schreiber. * The use of the iCycler is restricted to departmental personnel. ===== Fees ===== ^iCycler^ | $10/run | ===== Documentation ===== {{facilities:icycler_manual.pdf|iCycler Manual}}