iCycler Real-Time PCR System

The Bio-Rad iCycler iQ PCR detection system allows the user to both monitor and quantify a Polymerase Chain Reaction (PCR) in real time. A broad-spectrum tungsten light source and multiple filters permit a wide range of excitation and emission between 400-700nm, and also the multiplexing of up to four (4) different reporter fluorophors. On-line display allows visual confirmation of the PCR amplification in progress, and a 350,000 pixel CCD detector makes possible the simultaneous detection of up to 96 samples. The iCycler iQ is located in the Zigmond lab, room E734A. Please contact Rebecca Schreiber at x4615 for additional information.

• Dr. Richard Zigmond
  Professor
  368-4614
  rez@po.case.edu

• Rebecca C. Schreiber
  Training
  368-4615
  rcs519@case.edu

• Bio-Rad iCycler iQ real time PCR system.
• Dell 1.7 GHz Pentium PC with 512 MB RAM.
• Printer.

• All prospective users must attend a Bio-Rad training workshop prior to using the system.
• A copy of the user manual and software for data analysis is available through Rebecca Schreiber.
• Signup for the equipment is first come/first serve. The sign-up calendar is located on the door of room E734A.
• Data is stored on zip disks.
• The facility is not responsible for data storage and all data remaining on the hard drive is subject to immediate removal.
• The facility is not responsible for providing chemical reagents and consumable supplies. A list of the necessary reagents and supplies, however, may be obtained from Rebecca Schreiber.
• The use of the iCycler is restricted to departmental personnel.

iCycler Manual