====== Stefan Herlitze, Ph.D. ====== #template(faculty_profile.php|... * FName: Heather * LName: Broihier * Degree: Ph.D. * Picture: herlitze-1.jpg * Position: Assistant Professor * Department: Department of Neurosciences * Organization: Case Western Reserve University * Address_1: School of Medicine * Address_2: 10900 Euclid Ave * Address_3: Cleveland OH, 44106-4975 * Phone: (216) 368-1804 * Fax: (216) 368-4650 * Email: sxh106@case.edu * PubMed_Search: Herlitze S )# ===== RESEARCH INTERESTS ===== My lab is interested in understanding ion channel function and system modulation in animal physiology and behavior. Our research is centered on the characterization of voltage gated Ca2+ channels and their modulatory proteins in cerebellum and hindbrain. We will apply patch clamp measurements in cultured cells and slice preparations, virus mediated infection methods (Sindbis virus), imaging techniques (two photon microscopy, TIRF, FLIM, FRET, FRAP, BiFC) and mouse genetics (transgene expression and homologous recombination) to investigate the following topics: - //**Defining the role of P/Q-type Ca2+ channels domains for subcellular targeting, function and animal behavior.**// Ca2+ channels mediate voltage-dependent Ca2+ influx in subcellular compartments of neurons, triggering such diverse processes as neurotransmitter release, dendritic action potentials and excitation-transcription coupling. A fundamental question that remains unsolved is how Ca2+ channels are targeted to the appropriate cellular compartments especially the presynaptic terminal. This issue is critical to understanding both the basic physiology of neurons as well as several important neurological diseases such as familial hemiplegic migraine (FHM) and spinocerebellar ataxia 6 (SCA6), where mutations in voltage-gated Ca2+ channels cause changes in the density of Ca2+ channel complexes, alter their biophysical properties and change their potency to interact with intracellular modulating proteins like G proteins and Ca2+ channel ancillary subunits. We are in particular excited about investigating the role of the human P/Q-type SCA6 mutation in the living animal. The future goal of this research is to understand the mechanism of the disease process and to develop a cure. - //**Defining the role of G protein modulation and neuronal activity of the serotonergic transmitter system for animal physiology (blood pressure regulation) and behavior (anxiety and depression).**// The vertebrate serotonergic neurotransmitter system consists of a relatively small number of neurons, which project to the spinal cord and into almost every brain region. This expansive projection system thereby permits serotonergic neurons to modulate important physiological and behavioral functions such as mood, sexual behavior, circadian rhythm and anxiety. We are in particular interested in understanding how the regulation of G protein modulation within the serotonergic system influences the signaling output in the brain. This will ultimately lead to the understanding of mammalian behavior and may accelerate the development of therapeutic treatments for anxiety and depression. ===== SELECTED PUBLICATIONS ===== #template?key=Herlitze(dokudata.php|... * ID: 16306259 * Author: Li X, Gutierrez DV, Hanson MG, Han J, Mark MD, Chiel H, Hegemann P, Landmesser LT, Herlitze S. * Volume: 102 * Issue: 49 * Year: 2005 * Date: 2005 Dec 6 * Journal: Proc Natl Acad Sci U S A * Pages: 17816-17821 * Title: Fast noninvasive activation and inhibition of neural and network activity by vertebrate rhodopsin and green algae channelrhodopsin. * Status: 2005/11/23 [aheadofprint] * Selected: true ---- * ID: 16251278 * Author: Scott MM, Wylie CJ, Lerch JK, Murphy R, Lobur K, Herlitze S, Jiang W, Conlon RA, Strowbridge BW, Deneris ES. * Volume: 102 * Issue: 45 * Year: 2005 * Date: 2005 Nov 8 * Journal: Proc Natl Acad Sci U S A * Pages: 16472-16477 * Title: A genetic approach to access serotonin neurons for in vivo and in vitro studies. * Status: 2005/10/26 [aheadofprint] * Selected: true ---- * ID: 15824105 * Author: Li X, Hummer A, Han J, Xie M, Melnik-Martinez K, Moreno RL, Buck M, Mark MD, Herlitze S. * Volume: 280 * Issue: 25 * Year: 2005 * Date: 2005 Jun 24 * Journal: J Biol Chem * Pages: 23945-23959 * Title: G protein beta2 subunit-derived peptides for inhibition and induction of G protein pathways. Examination of voltage-gated Ca2+ and G protein inwardly rectifying K+ channels. * Status: 2005/04/11 [aheadofprint] * Selected: true ---- * ID: 15477380 * Author: Kurata HT, Phillips LR, Rose T, Loussouarn G, Herlitze S, Fritzenschaft H, Enkvetchakul D, Nichols CG, Baukrowitz T. * Volume: 124 * Issue: 5 * Year: 2004 * Date: 2004 Nov * Journal: J Gen Physiol * Pages: 541-554 * Title: Molecular basis of inward rectification: polyamine interaction sites located by combined channel and ligand mutagenesis. * Status: 2004/10/11 [aheadofprint] * Selected: true ---- * ID: 15000523 * Author: Herlitze S, Xie M, Han J, Hummer A, Melnik-Martinez KV, Moreno RL, Mark MD. * Volume: 35 * Issue: 6 * Year: 2003 * Date: 2003 Dec * Journal: J Bioenerg Biomembr * Pages: 621-637 * Title: Targeting mechanisms of high voltage-activated Ca2+ channels. * Selected: true ---- * ID: 14507926 * Author: Hummer A, Delzeith O, Gomez SR, Moreno RL, Mark MD, Herlitze S. * Volume: 278 * Issue: 49 * Year: 2003 * Date: 2003 Dec 5 * Journal: J Biol Chem * Pages: 49386-49400 * Title: Competitive and synergistic interactions of G protein beta(2) and Ca(2+) channel beta(1b) subunits with Ca(v)2.1 channels, revealed by mammalian two-hybrid and fluorescence resonance energy transfer measurements. * Status: 2003/09/24 [aheadofprint] * Selected: true ---- * ID: 11296298 * Author: Herlitze S, Zhong H, Scheuer T, Catterall WA. * Volume: 98 * Issue: 8 * Year: 2001 * Date: 2001 Apr 10 * Journal: Proc Natl Acad Sci U S A * Pages: 4699-4704 * Title: Allosteric modulation of Ca2+ channels by G proteins, voltage-dependent facilitation, protein kinase C, and Ca(v)beta subunits. * Selected: true ---- * ID: 11018106 * Author: Mark MD, Wittemann S, Herlitze S. * Volume: 528 Pt 1 * Issue: * Year: 2000 * Date: 2000 Oct 1 * Journal: J Physiol * Pages: 65-77 * Title: G protein modulation of recombinant P/Q-type calcium channels by regulators of G protein signalling proteins. * Selected: true ---- * ID: 10998041 * Author: Mark MD, Herlitze S. * Volume: 267 * Issue: 19 * Year: 2000 * Date: 2000 Oct * Journal: Eur J Biochem * Pages: 5830-5836 * Title: G-protein mediated gating of inward-rectifier K+ channels. * Selected: true ---- * ID: 10974320 * Author: Mark MD, Ruppersberg JP, Herlitze S. * Volume: 39 * Issue: 12 * Year: 2000 * Date: 2000 Sep * Journal: Neuropharmacology * Pages: 2360-2373 * Title: Regulation of GIRK channel deactivation by Galpha(q) and Galpha(i/o) pathways. * Selected: true ---- * ID: 10931840 * Author: Wittemann S, Mark MD, Rettig J, Herlitze S. * Volume: 275 * Issue: 48 * Year: 2000 * Date: 2000 Dec 1 * Journal: J Biol Chem * Pages: 37807-37814 * Title: Synaptic localization and presynaptic function of calcium channel beta 4-subunits in cultured hippocampal neurons. * Selected: true ---- * ID: 10332086 * Author: Herlitze S, Ruppersberg JP, Mark MD. * Volume: 517 ( Pt 2) * Issue: * Year: 1999 * Date: 1999 Jun 1 * Journal: J Physiol * Pages: 341-352 * Title: New roles for RGS2, 5 and 8 on the ratio-dependent modulation of recombinant GIRK channels expressed in Xenopus oocytes. * Selected: true ---- * ID: 9804555 * Author: Baukrowitz T, Schulte U, Oliver D, Herlitze S, Krauter T, Tucker SJ, Ruppersberg JP, Fakler B. * Volume: 282 * Issue: 5391 * Year: 1998 * Date: 1998 Nov 6 * Journal: Science * Pages: 1141-1144 * Title: PIP2 and PIP as determinants for ATP inhibition of KATP channels. * Selected: true ---- * ID: 9037084 * Author: Herlitze S, Hockerman GH, Scheuer T, Catterall WA. * Volume: 94 * Issue: 4 * Year: 1997 * Date: 1997 Feb 18 * Journal: Proc Natl Acad Sci U S A * Pages: 1512-1516 * Title: Molecular determinants of inactivation and G protein modulation in the intracellular loop connecting domains I and II of the calcium channel alpha1A subunit. * Selected: true ---- * ID: 8734989 * Author: Herlitze S, Villarroel A, Witzemann V, Koenen M, Sakmann B. * Volume: 492 ( Pt 3) * Issue: * Year: 1996 * Date: 1996 May 1 * Journal: J Physiol * Pages: 775-787 * Title: Structural determinants of channel conductance in fetal and adult rat muscle acetylcholine receptors. ---- * ID: 8637576 * Author: Herlitze S, Garcia DE, Mackie K, Hille B, Scheuer T, Catterall WA. * Volume: 380 * Issue: 6571 * Year: 1996 * Date: 1996 Mar 21 * Journal: Nature * Pages: 258-262 * Title: Modulation of Ca2+ channels by G-protein beta gamma subunits. * Selected: true ---- * ID: 8206920 * Author: Fakler B, Herlitze S, Amthor B, Zenner HP, Ruppersberg JP. * Volume: 269 * Issue: 23 * Year: 1994 * Date: 1994 Jun 10 * Journal: J Biol Chem * Pages: 16187-16194 * Title: Short antisense oligonucleotide-mediated inhibition is strongly dependent on oligo length and concentration but almost independent of location of the target sequence. ---- * ID: 7686380 * Author: Herlitze S, Raditsch M, Ruppersberg JP, Jahn W, Monyer H, Schoepfer R, Witzemann V. * Volume: 10 * Issue: 6 * Year: 1993 * Date: 1993 Jun * Journal: Neuron * Pages: 1131-1140 * Title: Argiotoxin detects molecular differences in AMPA receptor channels. ---- * ID: 1279705 * Author: Villarroel A, Herlitze S, Witzemann V, Koenen M, Sakmann B. * Volume: 249 * Issue: 1326 * Year: 1992 * Date: 1992 Sep 22 * Journal: Proc Biol Sci * Pages: 317-324 * Title: Asymmetry of the rat acetylcholine receptor subunits in the narrow region of the pore. ---- * ID: 1708143 * Author: Villarroel A, Herlitze S, Koenen M, Sakmann B. * Volume: 243 * Issue: 1306 * Year: 1991 * Date: 1991 Jan 22 * Journal: Proc Biol Sci * Pages: 69-74 * Title: Location of a threonine residue in the alpha-subunit M2 transmembrane segment that determines the ion flow through the acetylcholine receptor channel. ---- * ID: 2169445 * Author: Herlitze S, Koenen M. * Volume: 91 * Issue: 1 * Year: 1990 * Date: 1990 Jul 2 * Journal: Gene * Pages: 143-147 * Title: A general and rapid mutagenesis method using polymerase chain reaction. )# #template?query=Selected&key=Herlitze(publications.php|...)# #template?key=Herlitze(dokudata.php|...)# ~~NOTOC~~